Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following:
Exercise4: Purebacterialcolonies
Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?
Distinguishbetweena purecultureandamixedculture.
Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.
Whyshoulda Petridishnotbeleftopenforanyextendedperiod?
Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?
Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures
Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinicalspecimens.
Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?
Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?
Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?
Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?
Exercise3: Primarymediaforisolationofmicroorganisms
1.Definea differentialmediumanddiscussitspurpose.
2.Definea selectivemediumanddescribeitsuses.
3.WhyisMacConkeyagarselectiveas wellasdifferential?
4.Whyisbloodagarusefulasaprimaryisolationmedium?
5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?
Support your responses with examples.
Cite any sources in APA format
