Please carefully read the document I uploaded. It has all the information.
Once selected:
1. Use the Summary section/tab to obtain the gene name and information on the the
protein for you to describe its function (a short paragraph is fine).
2. Use the Structure section/tab to:
a) Obtain the alpha-carbon backbone of the structure of the protein.
b) Obtain the location of disease/clinical variants if there are any, if not show
population variants (i.e. amino acid variants).
3. Use the Tissue RNA Expression section/tab to:
a) Obtain a histogram of the tissue expression data for the gene.
b) Obtain a histogram on the cell type expression data in an organ where the gene is
highly expressed.
4. Use the Subcell section/tab to obtain immunofluorescence images on the distribution of
the protein in a cultured cell line (HeLa, Hep-G2 or U2OS) using the enlarged image o
a) Target protein only
b) target protein+nucleus
c) Target protein+nucleus+microtubules (more if you want to)
d) Then describe where the protein is found within the cell
5. Use the Tissue section/tab to:
a) Obtain an image of immunohistochemical staining for the protein in an organ tissue
that it is highly expressed (positive control).
b) Then describe where the protein is within the organ (cytoplasm, nucleus, or
membrane)
c) Obtain an image of immunohistochemical staining for the protein in an organ this it
is not expressed in (negative control).
d) Obtain an image for the distribution of the protein at the gross anatomical level in
either a biological female or male if it is not differentially expressed and if it is
differentially expressed, obtain an image of a male and a female.
6. Lastly, where are you going to go to find information on a protein from structure to
gross anatomy?
